Isolation and Characterization Of Multidrug Resistant E.coli and S.aureus From Selected Poultry Farms in Palestine
DOI:
https://doi.org/10.61856/drt2nx79Keywords:
MDR bacteria, Poultry, Antibiotics, Antibiotic resistant bacteria, Drinking water, Resistant, Sensitivity.Abstract
Poultry is one of the world’s fastest-growing sources of meat. As a result, antibiotics are increasingly being used to treat diseased hens and even to prevent infectious bacterial diseases, as well as growth promoters in diets at sub-therapeutic levels. This Inappropriate and indiscriminate usage of antibiotics results in the development of antibiotic-resistant bacteria. As a result, there is a growing public and governmental interest in phasing out inappropriate antibiotic usage in animal husbandry because of growing global worries that resistance bacteria can pass from animals to humans. There are concerns that these resistant bacteria will spread to communities around these enterprises. The presence of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in water sources and drinking water can harm poultry and human health, and it is a growing concern in the drinking water sector and a major public issue. Several antibiotic-resistant bacteria were found in poultry like Klebsiella, Shigella, E.coli, Staphylococcus aureus and epidermidis, Morexella, Neisseria, Clostridium, Salmonella, Brucella, Enterobacter, and Bordetella. Some of these bacteria are sourced from water but not others which may arise from other sources like soil, contaminated equipment, air, food, transported with flies, or direct contact with a diseased animal. However, in this study, we focused on MDR E.coli and S. aureus. The best antibiotics recommended for usage against the growth of bacteria were Ceftriaxone٫ Sulfamethoxazole and Ceftazidime because they produce synergism when used together.
References
Abdel-Tawab, A. A., Ammar, A. M., Nasef, S. A., & Reda, R. M. (2015). Prevalence of E. coli in diseased chickens with its antibiogram pattern. Benha Veterinary Medical Journal, 28(2), 224–230. https://doi.org/10.21608/bvmj.2015.32507
Apata, D. F. (2009). Antibiotic resistance in poultry. International Journal of Poultry Science, 8(4), 404–408. https://doi.org/10.3923/ijps.2009.404.408
Bolan, N. S., Stogy, A. A., Chuasavathil, T., Seshadri, B., Rothrock, M. J., & Panneerselvam, P. (2010). Uses and management of poultry litter. World’s Poultry Science Journal, 66(4), 673–698. https://doi.org/10.1017/S0043933910000656
Christian, A., Vivian, E., Boamah, C., Ngofi, Z., & Frank Boateng, O. (2018). Antibiotic use in poultry production and its effects on bacterial resistance. In Y. Kumar (Ed.), Antimicrobial Resistance – A Global Threat. IntechOpen. https://doi.org/10.5772/intechopen.79371
Clinical and Laboratory Standards Institute (CLSI). (2015). Performance standards for antimicrobial susceptibility testing; Twenty-fifth informational supplement (CLSI document M100-S25). Wayne, PA: CLSI.
Ejeh, F. E., Lawan, F. A., Abdulsalam, H., Mamman, P. H., & Kwanashie, C. N. (2017). Multiple antimicrobial resistance of Escherichia coli and Salmonella species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria. Sokoto Journal of Veterinary Sciences, 15(3), 45–53. https://doi.org/10.4314/sokjvs.v15i3.7
Galvin, S., Boyle, F., Hickey, P., Vellinga, A., Morris, D., & Cormican, M. (2010). Enumeration and characterization of antimicrobial resistant Escherichia coli bacteria in effluent from municipal, hospital, and secondary treatment facility sources. Applied and Environmental Microbiology, 76(14), 4772–4779. https://doi.org/10.1128/AEM.02898-09
Gyles, C. L. (2008). Antimicrobial resistance in selected bacteria from poultry. Animal Health Research Reviews, 9(2), 149–158. https://doi.org/10.1017/S1466252308001552
Hassam, Z. A., Shaw, E. J., Shooter, R., & Caro, D. (1978). Changes in antibiotic sensitivity in strains of Staphylococcus aureus, 1952–78. British Medical Journal, 2(6136), 536–537. https://doi.org/10.1136/bmj.2.6136.536
Khachatourians, G. G. (1998). Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. Canadian Medical Association Journal, 159(9), 1129–1136.
Khan, M. S., Muhammad, N. A., Haque, E., Barua, A., Chowdhury, T., Mullick, R., & Mahmud, A. M. (2014). Isolation and identification of nonplasmid multidrug resistant E. coli from poultry wastes in Chittagong region, Bangladesh. Journal of Bacteriology and Parasitology, 5(1). https://doi.org/10.4172/2155-9597.1000182
Lie, X., Payne, J. B., Santos, F. B., Levine, J. B., Anderson, K. E., & Shaleon, B. W. (2019). Salmonella populations and prevalence in layer feces from commercial high rise houses and characterization of the Salmonella isolates. United States Department of Agriculture.
Maddux, M. S. (1991). Effects of β-lactamase-mediated antimicrobial resistance: The role of β-lactamase inhibitors. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 11(2P2), 40S–50S.
Mamza, S. A., Egwu, G. O., & Mshelia, G. D. (2010). Antibiotic susceptibility patterns of beta-lactamase-producing Escherichia coli and Staphylococcus aureus isolated from chickens in Maiduguri (Arid zone), Nigeria. Veterinarski Arhiv, 80(2), 283–297.
Manie, T., Khan, S., Brözel, V., Veith, W., & Gouws, P. (1998). Antimicrobial resistance of bacteria isolated from slaughtered and retail chickens in South Africa. Letters in Applied Microbiology, 26(4), 253–258. https://doi.org/10.1046/j.1472-765X.1998.00312.x
Miles, T. D., McLaughlin, W., & Brown, P. D. (2006). Antimicrobial resistance of Escherichia coli isolates from broiler chickens and humans. BMC Veterinary Research, 2(1), 7. https://doi.org/10.1186/1746-6148-2-7
Miranda, J. M., Vazquez, B. I., Fente, C. A., Barros-Velázquez, J., Cepeda, A., & Franco, C. M. (2008). Evolution of resistance in poultry intestinal Escherichia coli during three commonly used antimicrobial therapeutic treatments in poultry. Poultry Science, 87(8), 1643–1648. https://doi.org/10.3382/ps.2007-00485
Moustafa, S., & Mourad, D. (2015). Resistance to 3rd generation cephalosporin of Escherichia coli isolated from the feces of healthy broilers chickens in Algeria. Journal of Veterinary Medicine and Animal Health, 7(8), 290–295. https://doi.org/10.5897/JVMAH2015.0396
Naseer, B. S., & Jayaraj, Y. (2010). Identification of multiresistant Staphylococcus aureus in clinical specimens. Research Journal of Medical Sciences, 4(2), 204–207. https://doi.org/10.3923/rjmsci.2010.204.207
Ngogang, M. P., Ernest, T., Kariuki, J., MouliomMouiche, M. M., Ngogang, J., Wade, A., & van der Sande, M. A. B. (2021). Microbial contamination of chicken litter manure and antimicrobial resistance threat in an urban area setting in Cameroon. Antibiotics, 10(1), 20. https://doi.org/10.3390/antibiotics10010020
Nichols, G. L. (2005). Fly transmission of Campylobacter. Emerging Infectious Diseases, 11(3), 361–364. https://doi.org/10.3201/eid1103.040460
Olonitola, O. S., Fahrenfeld, N., & Pruden, A. (2015). Antibiotic resistance profiles among mesophilic aerobic bacteria in Nigerian chicken litter and associated antibiotic resistance genes. Poultry Science, 94(5), 867–874. https://doi.org/10.3382/ps/pev069
Rizzo, L., Manaia, C., Merlin, C., Schwartz, T., Dagot, C., Ploy, M. C., & Fatta-Kassinos, D. (2013). Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: A review. Science of the Total Environment, 447, 345–360. https://doi.org/10.1016/j.scitotenv.2013.01.032
Romanus, I. I., Chinyere, O. E., & Amobi, N. E. (2012). Antimicrobial resistance of Escherichia coli isolated from animal and human clinical samples. Global Research Journal of Microbiology, 2(1), 85–89.
Salihu, A. E., Onwuliri, F. C., & Mawak, J. O. (2014). Antimicrobial resistance profiles of Salmonella gallinarum isolates from free range chickens in Nasarawa State, Nigeria. International Journal of Bacteriology Research, 2(1), 19–27.
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