Treatments of Coleus forskholii that increase rooting to get larger crude forskolin extract

Abstract

The aim is study of effect of some phytohormones and microelements in a tissue culture system to extract forskolin by different solvents from hairy roots in Plectranthus barbatus Andr. (Syn.Coleus forskohlii Briq.). Our results indicated that, the best parameters for increasing the number and length of roots were by indole butyric acid. Low concentrations of boron and silicon also promote rooting of Coleus cuttings. Methanol is a more efficient solvent than ethanol and ethyl acetate in forskolin extraction. Root hairs of the terminal cuttings of Coleus forskohlii appear after treatment with bacterial inoculum Agrobacterium rhizogenes. Forskolin levels increased to 13 and 5.3 mg/L in hair roots after 4 weeks of incubation. Forskolin extraction from tissue culture media is more efficient (40 mg/L per g wet weight) compared to extraction from root cuttings (13 mg/10 g wet weight).