Evaluation the role of laminin α1 in wound healing of skin mice by using Immunohistochemical assessment.
DOI:
https://doi.org/10.61856/zgwwkt17Keywords:
wound healing injury laminin α1 skinAbstract
Wound healing is a process through which the skin and subcutaneous tissue restore itself after it has been injured wound healing can be illustrated in a progression of physical characteristics (phases) that constitute the post-trauma repairing process. Aim: To know and evaluate the role of laminin α1 during the process of wound healing in the skin according to days of wound injuries. Materials & Methods: Thirty-two male mice, about 3-6 months, were divided into four groups according to days of wound injury. Each group has eight mice. Group (A) collects specimens on the first day of injury. Group (B) after one week of injury. Group (C) after two weeks. Group (D) control group collects specimens without injury. Tissue Sections were treated with laminin α1. Used Aperio Scope software to assess immunohistochemical reactivity. For statistical analysis used SPSS program. Results: immunohistochemical study reveals group (A) animals a very low expression laminin α1 at (0.15±0.008pixel/(micron)2) compared with group (B) showing high expression laminin α1 after one week at (0.69±0.02pixel/(micron)2). group (C) show week expression laminin α1 after two weeks (14day) at (0.39±0.02pixel/(micron)2). In group (D) (control group) expression of laminin α1 at (0.36±0.01pixel/(micron)2). Conclusion: the laminin α1 protein has an important role during injury healing, through significantly elevated lamininα1in the first week and beginning to descend at the end of the second week. Can be used this timing as a guideline for injection or topical use of laminin α1 to promote wound healing after injury.
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