A histochemical study to evaluate the role of some isoindoline-1-one compounds γ-lactam compounds on the biological functions of adult laboratory rabbits

Abstract

This study is concerned with the synthesis and characterization of derivatives of the isoindoline-1-one γ-lactams (c-d), were prepared by reacting Phenyl succinic anhydirde with the appropriate Schiff base (a-b), in the presence of γ-lactam compounds were prepared from the reaction between 2-chloro benzoyl chloride  with imines (a-b) in the prescence Phenyl lithium  under (N2) atm (-5) in THF solvent to yielded product γ-lactams. reaction was verified by thin layer chromatography (TLC), and the formed compounds were identified by NMR, IR, mass spectra. The  proposed  reaction  for  their  formations . In the current study, 30 white male rabbits with an average weight of 250 g were used, they were randomly divided into three groups as follows, group (1) includes 10 untreated rabbits as a control group, group (2) consists of 10 rabbits treated with the first compound. A) at a concentration of 30 mg | kg | day, group (3) consisted of 10 rabbits that were treated with the second compound (B) at a concentration of 30 mg | kg | day. The dose was determined based on body weight and all animals received the doses by oral administration for thirty days Blood samples were taken at the end of the experiment by a direct heart stab and the samples were centrifuged, and they were kept in special tubes at 4°C until the start of measuring the industrial and enzyme functions of the kidney. % of all mice until the start of preparing the tissue sections and studying the effect of the two compounds. The two compounds varied in their effect on some biochemical parameters related to the manufacturing and enzyme function of the kidney. Histologically, the results indicated that treatment with compound A did not show any histological changes, while the results of treatment with compound B showed enlargement of some kidney cells and atrophy of the nuclei of other cells with cases of fibrosis, blood congestion and infiltration of inflammatory cells compared to control group.